Expression of NDRG2 and AKT2 protein and their phosphorylated forms in gliomas

Objective To investigate the expression levels of N-myc downstream regulated gene 2 (NDRG2) and serine/threonine kinaseβ2 (AKT2) protein and their phosphorylated forms in different pathological grades of glioma samples.Methods Glioma samples inⅡ,Ⅲ,orⅣpathological grade were collected and total protein was extracted respectively.Then Western blot analysis was applied to detect the expression levels of NDRG2 and AKT2 protein and their phosphorylated rotors in all samples.All data were analyzed.Results There was clear negative correlation between the expression of NDRG2 protein and the glioma tumor grade (P0.05).The expression level of two kinds of phosphorylated NDRG2 protein was high in most of samples in which the phosphorylated AKT2 slightly expressed or did not express,and the expression of two kinds of phosphorylated NDRG2 protein was slight in most of samples where the phosphorylated AKT2 highly expressed. Conclusion The expression level of NDRG2 in gliomas correlates with the pathological grade.The phosphorylation of NDRG2 does not depend on the phosphorylation of AKT2 and a competitive mechanism maybe exists in the two processes.