Preparation method of phosphorylated epimedium polysaccharide

The invention belongs to the technical field of preparation and structural transformation of traditional Chinese medicine polysaccharide and relates to a preparation method of phosphorylated epimedium polysaccharide. The preparation method of the phosphorylated epimedium polysaccharide comprises the following steps: carrying out heating reaction on a phosphorylating reagent (sodium tripolyphosphate-sodium trimetaphosphate), epimedium polysaccharide and sodium sulfate for a period of time under alkaline condition, carrying out phosphorylation molecular modification, and selecting out optimal conditions for phosphorylation modification on the epimedium polysaccharide by carrying out a response surface experiment with content of phosphate radicals of products and reagent ratio, reaction temperature, reaction pH value and reaction time as factors. After the phosphorylated epimedium polysaccharide is prepared, in vitro activity of a modified product is studied, and results show that the phosphorylated epimedium polysaccharide has a certain immunoregulation, antiviral and antioxidant effects.