Seed cell screening and culturing cryopreservation technical method of human amniotic mesenchymal stem cells for tissue engineering

The invention discloses a seed cell screening and culturing cryopreservation technical method of human amniotic mesenchymal stem cells for tissue engineering, and belongs to the field of biological stem cell medical medicines. The amniotic mesenchymal stem cells are derived from multipotential stem cells of mesoblast. The invention describes and particularly discloses an innovative stem cell acquisition, separation, extraction and cryopreservation technique and a placenta amniotic layer high-simulation in-vivo extracellular matrix acquisition and preparation technique, the techniques are significant points of innovative techniques of hAMSCs adhesion extraction and preparation, multi-combined culture liquid gradient serum-free habituated culture system design is also a key technique needed to particularly acquire hAMSCs, and the key technique combined extraction culture is efficient bionic biotechnology invention. The acquired stem cells are accordant with the international society for cellular therapy (ISCT) standard and has high purity, stem cell proliferation amplification and good cell activity, meanwhile design and development of future PDB-MSCs stem cell clinical medicines can be met, stem cell recycling requirements are provided in medical rescue, and multipurpose requirements of seed stem cells needed to make tissue engineering skin are provided.