Effects of lymphocytes defect on activation of peritoneal macrophages in mice with endotoxemia

This study aimed to investigate the effects of lymphocytes defect on activation of peritoneal macrophages in mice with endotoxemia.Endotoxemia models of Balb/c mice and severe combined immunodeficiency(SCID) mice were established by injecting LPS intraperitoneally.Peritoneal fluids of both mice were collected for testing peritoneal TNF-α and IL-10 by ELISA,while peritoneal macrophages(PMa) were used for testing TNF-α,IL-10 and mitogen-activated protein kinase phosphatase-1(MKP-1) mRNA expression by real-time PCR.PMa from both mice were stimulated by LPS in vitro,and supernatants were tested for TNF-α and IL-10 by ELISA.For SCID mice,the levels of peritoneal TNF-α were higher at 1 h but the levels of peritoneal IL-10 were lower at 3 h after LPS injection,as compared with Balb/c mice.TNF-α mRNA expression of PMa from SCID mice was higher but IL-10 mRNA expression was lower than those of PMa from Balb/c mice after and even before LPS injection.After LPS stimulation in vitro,PMa from SCID mice secreted more TNF-α but less IL-10,as compared with PMa from Balb/c mice.MKP-1 mRNA expression of PMa from Balb/c mice were higher than those from SCID mice before and after LPS injection.Altogether,with defective lymphocytes,activities of PMa increase in SCID mice with endotoxemia.Lymphocytes suppress macrophages activation and may regulate macrophages' development,and they may act through increasing MKP-1 expression of macrophages.