The validation experiments of Methylene blue virus inactivation/removal process on swine thrombin

Purpose To test the result of methylene blue virus inactivation/removal process on swine thrombin and to improve the production process.Methods Adding 0.5 mg/L methylene blue on Sindbis virus,of EMCV and PPV and PRV viruses,then putting them in the inactivated virus illumination instrument for 2 hours and using 60 kD ultrafiltration method to validate the feasibility of the process to inactivated virus.Results Methylene blue inactivated and filter virus could effectively remove the virus by ultrafiltration.Viruses which were incorporated into thrombin and in the original culture,lasty lose the ability to cell infection,and reduce the TCID50 higher than 4Log10.Conclusion Methylene blue virus inactivation/removal process can effectively inactivate the virus in swine thrombin.