Vybrant DyeCycle Violet Stain Discriminates Two Different Subsets of CD34+ Cells
2016
Introduction: Studies are needed to understand the role of CD34 expressing cells with regard
to efficient engraftment, especially in the adjuvant treatment of cancer. Materials and Methods: In
this study we have used a modified method in our laboratory for routinely counting CD34+ cells. Unlysed
whole blood samples were stained with the DNA-selective and cell membrane-permeant Vibrant
DyeCycle Violet stain. Results: CD34+ cells exhibit a consistent and differential Vybrant Dye Cycle
Violet staining pattern. Based on their different DCV intensity, we classified these subpopulations as
CD34+/DCV high and CD34+/DCV low cells. In general, DCV high cells are about 12-times brighter than DCV low cells.
Conclusion: DCV staining may be used to discriminate subsets of CD34+ cells similarly to other methods which have
previously defined different functional properties that can be related to the characterization, resolution, and purification of
primitive hematopoietic stem cells in combination with specific useful markers for multicolor flow cytometric measurements.
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