Effects of hypoxia and reoxygenation of the conversion of xanthine dehydrogenase to oxidase in Chinese hamster V79 cells

1991 
Abstract The effects of hypoxia and reoxygenation on the conversion of xanthine dehydrogenase of the free radical-producing xanthine oxidase in Chinese hamster V79 cells have been investigated using a newly developed fluorimetric enzyme assay. Hypxia caused an increase in xanthine oxidase activity from 25% to 80% of the total activity of xanthine oxidase and dehydrogennase. The ratio returned to normal levels within 24 h of aerobic incubation. Hypoxia caused the release of xanthine oxidase in the medium of V79 cells and an increase in total protein concentration in the medium. There was an early change induced in lipid peroxidation markers and this was inhibited by allopurinol. The effects of glucose deprivation and calcium blockers were also inevestigated. Fura-2 AM was found to interact with V79 cell, making it impossible to determine intracellular calcium levels in v79 cells by this reagnet.
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