Immunoscreening of cDNA Library of Adult Trichinella spiralis

cDNA library of 3 days old adult of Trichinella spiralis was screened using the swine sera artificial infected with Trichinella spiralis and the positive clones were sequenced and analysed. From 4.5×10~5 recombinant, 33 positive clones were obtained. Sequence analysis revealed 16 new genes and 4 known genes, positive clones Zh8, 9, 10, 12, 13, 14, 20, 26, 28, 29, 36, 54, 68, 70 encoded serine protease. This will lay the foundation to further study on the Trichinella spiralis antigen genes.