Immunoscreening

Immunoscreening is a method of biotechnology to detect a polypeptide produced from a cloned gene. The term encompasses several different techniques designed for protein identification, for example Western blotting. Immunoscreening is a method of biotechnology to detect a polypeptide produced from a cloned gene. The term encompasses several different techniques designed for protein identification, for example Western blotting. Clones are screened for the presence of the gene product (a protein).This strategy requires first that a gene library is implemented in an expression vector, and that antiserum to the protein is available.Radioactivity or an enzyme is coupled generally with the secondaryantibody.The radioactivity/enzyme linked secondary antibody can be purchased commercially and can detect different antigens.In commercial diagnostics labs, labelled primary antibodies arealso used.