Urea as the best fibrin solubilizer in the thrombin inhibition analysis: Theoretical and experimental modeling of fibrinogen denaturation

Abstract Due to worldwide thrombosis incidence, new anticoagulant drugs have been developed. Therefore, diverse techniques are used to measure this capacity. In this sense, a size-exclusion chromatography method has already proposed, which depends on the fibrin solubilization step. Thus, the work aimed to establish the influence of different fibrin solubilizers on fibrinogen denaturation through theoretical-experimental modeling. Theoretical results showed that the coupling energy for fibrinogen-TCA is lower than that obtained for fibrinogen-urea and fibrinogen-bromide coupling, while experimental results evidenced partial fibrinogen precipitation with TCA, NaBr, and urea, resulting this last the best solubilizer to measure thrombin inhibition.