Substrate specificity of haloalkane dehalogenases
2011
An enzyme's substrate specificity is one of its most important
characteristics. The quantitative comparison of
broad-specificity enzymes requires the selection of a
homogenous set of substrates for experimental testing,
determination of substrate specificity data, and analysis using
multivariate statistics. We describe a systematic analysis of
the substrate specificities of nine wild-type and four
engineered haloalkane dehalogenases. The enzymes were
characterized experimentally using a set of 30 substrates
selected using statistical experimental design from a set of
nearly 200 halogenated compounds.
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