In silico characteration of GmSOS1 provides a comprehensive understaning for its role in soybean salt tolerance
2020
Plant SOS1 gene encodes plasma membrane Na+/H+ antiporter, which helps in the exclusion of Na+ and improves plant salt tolerance. However, detailed studies of SOS1 gene in the important oil crop, soybean (Glycine max), are still lacking. In the present study, we carried out a comprehensive bioinformatics analysis of SOS1 gene in soybean. Referring to the analysis of physicochemical properties and structural characteristics, the GmSOS1 is an acidic protein with instability and hydrophobicity. Subcellular localization of GmSOS1 supports our presumption that GmSOS1 protein is a plasma membrane Na+/H+ antiporter. The prediction of phosphorylation sites indicates 4 sites that may be activated. Further, the functional protein association network and co-functional network signify the potential role of GmSOS1 in salt stress tolerance. Although the interaction between GmSOS1 and GmHKT1 remains elusive, some of the intermediary signaling components of SOS pathway in soybean have been predicted. In addition, in silico expression analysis based on transcriptome datasets using publicly available database revealed that GmSOS1 was differentially expressed in tissues and different times. Due to the analysis of its regulation mechanism, we found transcription factors such as WRKY and ERF as well as three miRNAs can regulate the expression of GmSOS1. Phylogenetic analysis using the homologous amino acid sequence of SOS1s from 26 species was performed to study the conserved motifs among these SOS1 members. Overall, we provide an extensive analysis of the GmSOS1 gene and it promises the primary basis for the study in development and response to salt tolerance.
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