Patkány fogbél eredetű őssejtek tenyésztése és differenciáltatása szövetépítési kutatások megalapozására

Although existence of dental stem cells has been known since 2000, and these are already well characterized, hardlyany data are available regarding rat dental pulp cells. The aim of our study was to isolate, cultivate and differentiate primarycell cultures, showing stem cell properties, from rat incisor pulp. Cell viability was determined using the WST-1test. Osteogenic differentiation potential was examined by von Kossa and Alizarin Red staining, and the neurogenic potentialby morphological studies, immunocytochemistry and quantitative PCR. Moreover, the effect of different surfacecoatings on neurogenic differentiation was also investigated. We have succeeded in isolating stem cell-containing cellcultures from the pulp tissue of rat lower incisors and optimized the culture conditions. We have provided evidence thatthese cell cultures are capable for differentiating towards both osteogenic and neurogenic lineages. We have developeda novel neurogenic differentiation protocol, which is more effective than the previous one. Lysine-laminin and ornithinlaminincoated surfaces were found to be better for rat pulp-cell differentiation. Our results confirm that dental-pulp stemcells are capable of regenerating injured bone and neural tissue. Our model provides the foundation for the clinical useof dental pulp stem cells for tissue engineering purposes.