Sensitive detection of Interleukin-6 (IL-6) on the plasmonic chip by Grating Coupled-Surface Plasmon-field enhanced Fluorescence Imaging

As for a sensitive immunosensor, we focused on the Surface Plasmon-field enhanced Fluorescence Spectroscopy (SPFS) based on the Surface Plasmon Resonance (SPR) in some powerful techniques. Recently, we have sensitively detected analytes with Grating Coupled-Surface Plasmon-field enhanced Fluorescence Imaging (GC-SPFI). In this study, Interleukin-6 (IL-6), which acts as both pro-inflammatory and anti-inflammatory cytokine, was detected on the plasmonic chip by the GC-SPFI instrument. IL-6 was quantitatively evaluated at low concentration due to the effect of fluorescence enhancement.