Determination of Hepatocellular Carcinoma Serum Polypeptide Based on Antibody Pre-concentration Coupled with Mass Spectrometry

Abstract Polypeptide antibody pre-concentration method coupled with mass spectrometry have been established for determination of serum polypeptide. Polypeptide was captured by agarose beads followed by binding of specific polyclonal antibodies from serum. After timed washing steps, the remaining bound polypeptides were eluted from the beads and detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The ion signals of protein A agarose bead-based platform amenable to high throughput peptide capture followed by mass spectrometry were achieved with enough precision (RSD