The in vitro synthesis of 2′-omethylguanosine and 2-methylthio 6N (γ,γ, dimethylallyl) adenosine in transfer RNA of Escherichia coli

Abstract A cell-free extract of E. coli catalyzes the transfer of the methyl group from S-adenosylmethionine 1 to the 2′OH group of a guanosine residue present in a GG sequence in transfer RNA tyr . SAM is also shown to serve as a methyl donor in vitro for the synthesis of 2-methylthio 6 N (γ,γ, dimethylallyl) adenosine in transfer RNA.