The Effects of Eosinophils Activated with Airborne Fungi on Nasal Epithelial Cells

The eosinophils are involved in physiologic and pathologic process, such as asthma, parasiticdisease, and nasal diseases. In chronic rhinosinusitis (CRS), most of the eosinophils in nasal cavity were activated with extracellulardeposition of granule proteins. The nasal epithelial cells are an active participant in airway inflammation. The aim of thisstudy is to know the influence of activated eosinophils on nasal epithelial cells. Subjects and Method：Eosinophils wereisolated from healthy volunteers and stimulated with three common fungi (Alternaria, Aspergillus, and Bipolaris) to obtainconditioned media (EoCM). Nasal epithelial cells were obtained from CRS patients with polyps and cultured with EoCM for 48hours. Interleukin-6 (IL-6), IL-8, and granulocyte-macrophage colony stimulating factor (GM-CSF) were measured to determinethe activation of epithelial cells. Results：Nasal epithelial cells stimulated with EoCM produced larger amount of IL-6,IL-8 and GM-CSF than negative controls ( p