Imipramine and citalopram reverse corticosterone-induced alterations in the effects of the activation of 5-HT1A and 5-HT2 receptors in rat frontal cortex

Using extracellular recording we studied changes in the reactivity of rat frontal cortical slices to the 5-HT 1A , 5-HT 2 and 5-HT 4 receptor agonists, (+)-2-dipropyloamino-8-hydroxy-1,2,3,4-tetrahydronaphtalene hydrobromide (8-OH-DPAT), (±)-2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI) and zacopride, respectively, induced by an earlier treatment of animals with corticosterone lasting 1 or 3 weeks. Spontaneous bursting activity was recorded in ex vivo slices incubated in a medium devoid of Mg 2+ ions and containing picrotoxin (30 μM). Repetitive, but not single, corticosterone administration resulted in an attenuation of the effect of the activation of 5-HT 1A receptors and in an enhancement of the effect related to 5-HT 2 receptors. The effect of 5-HT 4 receptor activation remained unchanged. In separate two sets of experiments rats were treated with corticosterone for 3 weeks and additionally with imipramine or citalopram, beginning on the eighth day of corticosterone administration. In the corticosterone plus imipramine as well as corticosterone plus citalopram groups the effects of 8-OH-DPAT and DOI were not different from control indicating that corticosterone-induced functional modifications in the reactivity of 5-HT 1A and 5-HT 2 receptors were reversed by antidepressant treatments.