OPTIMIZED METHOD FOR OBTAINING CHITOSAN NANOPARTICLES LOADED WITH XANTHINE DERIVATIVE

The use of nanotechnology is the current trend for drug delivery approaches. Aim: This research was focused on the development of an optimized method for preparation and characterization of chitosan nanoparticles (CSNPs) and xanthine derivative loaded CSNPs. Materials and methods: In order to develop appropriate method different parameters such as the concentration of chitosan and tripolyphosphate as well as the stirring speed were varied. The aggregation of the nanoparticles (NPs) was prevented by using Tween 80 as a surfactant. Results: Following the CSNPs optimization process nanoparticles in the range of 231-244 nm were obtained. The optimized method was used for the loading of xanthine derivative in the CSNPs. The particle size of CSNPs loaded with xanthine derivative was 267 nm and the loading efficiency was ~ 86%. In presence of the surfactant, the NPs size decreases slightly from 267 nm to 193 nm with increasing the concentration of Tween 80. The CSNPs loaded with xanthine derivative remain stable after 72 h of storage. Conclusions: The CSNPs and xanthine derivative loaded CSNPs obtained after the optimization method by modifying several independent variables can be characterized as having small size and a good stability over time. Given these important features CSNPs are promising materials for targeted drug delivery system.