CRISPR‐S: an active interference element for a rapid and inexpensive selection of genome‐edited, transgene‐free rice plants

2017 
The CRISPR/Cas9-based genome editing tool has been used in diverse applications related to plant research, including for crop improvement (Sun et al., 2016; Liu et al., 2017). Mutant plants may be generated via transient transformations or DNA-free editing (Liang et al., 2017). However, plant genomes are often edited during the production of transgenic plants, in a process that involves the identification of targeted edits in regenerated T0 plants and the subsequent elimination of transgenes in T1 plants (Sun et al., 2016). This article is protected by copyright. All rights reserved.
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