Novel vip3-like gene killing Lepidoptera insects, and its application

The invention relates to agricultural pests, especially relates to materials and methods of Lepidoptera pests having serious damage to agricultural production, and discloses a novel vegetative insecticidal protein gene vip3-like gene obtained through separating and cloning from Bacillus thuringiensis wild strain BtHS66. A toxin protein coding the gene is composed of 942 amino acid residues, and has a homology of below 30% with a present disclosed protein sequence. The novel vip3-like protein has an efficient insecticidal activity against Lepidoptera insect diamondback moth, and the novel vip3-like gene can be used for constructing gene engineering strains or cultivating transgenic crops, so the insecticidal spectrum is broadened, and the resistance of pests to Cry1 and Cry2 proteins widely used at present can be delayed.