Identification of Cuts-specific Myogenic Marker Genes in Hanwoo

Department of Parasitology, College of Medicine, Inje University Busan, Republic of KoreaABSTRACTMyogenic satellite cells(MSCs) are mononuclear, multipotent progenitors of adult skeletal muscle possessing a capacity of forming adipocyte-like cells(ALC). To identify the skeletal muscle type-specific myogenic and adipogenic genes during MSCs differentiation, total RNA was extracted from bovine MSCs, myotube-formed cell(MFC), and ALC from each of Beef shank, Longissimus dorsi, Deep pectoral, and Semitendinosus. DNA microarray analysis(24,000 oligo chip) comparing MSCs with MFC and ALC, respectively, revealed 135 differentially expressed genes(>4 fold) among four cuts. Real-time PCR confirmed expression of 29 genes. Furthermore, the whole tissue sample RNAs analysis showed 6 differentially expressed genes in Beef shank. Among which, 1 gene in MSCs, 4 in MFC, and 1 in ALCs were highly expressed. This study will provide an insight for better understanding the molecular mechanism of differentiation of skeletal muscle type-specific MSCs. The identified genes may be used as marker to distinguish skeletal muscle types. (Key words : Skeletal muscle type-specific myogenic and adipogenic genes, Hanwoo, DNA marker, Myogenic stem cell, Muscle cell)