The mapping of the local content of water and dry matter by using ultrathin frozen sections

The possibility and applications of quantitative dry mass and water content mapping using an image analysis of darkfield intensity digitalization were shown on ultrathin freeze-dried cryosections. The distribution map obtained may be further used both for direct measurements of local dry mass and water content under physiological state and pathological disorders and for calculation of the concentration values obtained by quantitative X-ray mapping on freeze-dried cryosections in terms of millimoles per litre of cellular water.