The increased expression of TCF3 is correlated with poor prognosis in Chinese patients with nasopharyngeal carcinoma

Objectives Regulatory factors controlling stem cell identity and self-renewal are often active in aggressive cancers and are thought to promote cancer growth and progression. B-cell-specific transcription factor 3 (TCF3/E2A) is a member of the T-cell factor/lymphoid enhancer factor (TCF/LEF) transcription factor family that is central to regulating epidermal and embryonic stem cell identity. It has been reported that TCF3 was connected with the development and progression of a number of human cancers. In this study, we aimed to identify the expression of TCF3 in human NPC and evaluate its clinical significance. Design To investigate the expression of TCF3 in nasopharyngeal carcinoma (NPC) and its relationship to prognosis. Setting An in vitro study. Main outcome measures We analyzed the expression of TCF3 in NPC and in non-tumorous nasopharyngeal tissues by quantitative RT-PCR and Western blotting. The expression patterns of TCF3 in 117 archived paraffin-embedded NPC specimens were characterized by immunohistochemistry (IHC), and the correlation between of the TCF3 protein expression and the clinicopathological features of NPC was analyzed. Results We observed that TCF3 had a higher expression in nasopharyngeal carcinoma than in non-tumorous nasopharyngeal tissues of 117 archived paraffin-embedded NPC specimens, and 80 (68.4%) biopsy tissues revealed high levels of TCF3 expression. Furthermore, statistical analyses demonstrated that the increased expression of TCF3 was closely related to clinical stage, locoregional recurrence and distant metastasis of NPC. NPC patients with high levels of TCF3 expression had a shorter survival time, whereas patients with lower levels of TCF3 expression survived longer. Moreover, multivariate analysis suggested that the upregulation of TCF3 was a critical prognostic factor for NPC. Conclusions Our observations suggest, for the first time, that TCF3 is significantly associated with the development and progression of NPC, which can be used as an important prognostic marker for NPC patients and may be an effective target for the treatment of NPC. This article is protected by copyright. All rights reserved.