Effects of exercise and resveratrol on visceral fat resistin expression and plasma resistin concentration in elderly obese rats

OBJECTIVE: To exploer effects of exercise and resveratrol on visceral fat resistin expression and plasma resistin concentration in elderly obese rats. METHODS: SD rats as three stage of fattening divided the growth period, middle-aged and elderly, toestablish rats model of elderly obesity. 6 natural growing aged rats were selected as blank control group( C). 24 elderly obese rats were randomly divided into 4 groups, 6 rats in each group: obese control group( OC), supplementary resveratrol group( OR), exercise group( OE), exercise combined with resveratrol group( OER). Running speed and time of animal were 15 m/min×15 min for each group, 4 groups/times, rest between groups5 min, 60min/time. For resveratrol treatment, each rat was fed 52. 5mg/kg, one time/day, 5 times/week, for 8 weeks. The content of blood glucose was measured, the insulin resistance was calculated, testing the expression of resistin mRNA in visceral adipose tissue and plasma Resistin concentration. RESULTS: Body weight, visceral fat mass, body fat ratio, and blood glucose, and insulin resistance of OC group were higher than C group( P < 0. 01, P < 0. 05), Resistin mRNA×10~3 expression in visceral adipose tissue and plasma concentration of OC group(( 29. 80 ± 4. 91) μg/L and( 4. 30 ± 1. 93) μg/L, repectively) were higher than C group(( 23. 09 ± 6. 12) μg/L and( 3. 49 ± 0. 42) μg/L, repectively), but the difference was not statistically significant. Body weight, visceral fat mass, body fat rate, and blood glucose, and insulin resistance of OE group and OER group were lower than OC group( P < 0. 01, P < 0. 05), of OR group were lower than OC group, of OER group were lower than OE group, but the difference was not statistically significant. Resistin mRNA×10~3 expression in visceral adipose tissue of OR group( 21. 63 ±4. 91) was lower than OC group( P < 0. 05), of OE group and OER group(( 28. 21 ± 4. 80), ( 25. 22 ± 11. 12), respectively) were lower than OC group, but the difference was not statistically significant. Plasma resistin concentration of OR group and OE group and OER group(( 3. 71 ± 1. 01), ( 3. 29 ± 1. 19) and( 2. 99 ± 0. 44) μg/L, respectively), were lower than OC group, of OER group were lower than OE group, of OE group were lower than OR group, but the difference was not statistically significant. CONCLUSION: Elderly obesity result in insulin resistance and high blood glucose, resistin expression in visceral adipose tissueand and plasma resistin concentration is higher. In elderly obese rats, simple exercise and exercise combined with resveratrol can significantly reduce weight, visceral fat mass, body fat ratio, and blood glucose content, and insulin resistance, but that are less affected on resistin expression in visceral fat and plasma resistin concentration. Simple add resveratrol can reduce resistin mRNA expression in visceral adipose tissue.