cell culture in alginate hydrogel spheres chitosan

A method of producing a hydrogel matrix comprising cartilage-forming cells, said method comprising the steps of: - providing a solution of alginate, - provide a solution with chitosan having less than 60 kDa Pm, - mixing the solution of alginate and chitosan solution with cartilage-forming cells, wherein said mixed solution comprises between 0.5 and 0.7% (w / v) of chitosan and between 1 and 1.4% alginate (p / v), - introducing the mixed solution droplets in a solution containing cations or Sr2 Ca2 + and - isolating polymerized areas of the solution with cations.