Fluorescent dye containing high-energy phosphate bond

The invention discloses a fluorescent dye containing a high-energy phosphate bond. The fluorescent dye has a structure in a general formula I in figure 1. The fluorescent dye has a relatively low fluorescent background in non-mitochondria of a living cell, has a relatively high fluorescence signal in a mitochondria region in the living cell, and has a strong specificity mark on adenosine triphosphatase in the living cell. The compound has a certain level of water solubility, and has good cell membrane permeability. The compound also has relatively low biotoxicity, phototoxicity and photobleaching property; a large enough difference exists between the spectral range of the fluorescent dye and the spectral range of a biological sample.