In enzymology, a triphosphatase (EC 3.6.1.25) is an enzyme that catalyzes the chemical reaction In enzymology, a triphosphatase (EC 3.6.1.25) is an enzyme that catalyzes the chemical reaction Thus, the two substrates of this enzyme are triphosphate and H2O, whereas its two products are diphosphate and phosphate. This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is triphosphate phosphohydrolase. This enzyme is also called inorganic triphosphatase.