In-vitro evaluation of rutin and rutin hydrate as potential radiation countermeasure agents

Background : DNA damage is one of the major consequences of radiaon exposure onto the biological systems. A series of compounds including flavanoids were found to render DNA protecon against radiaon damage. In this study we elucidated the potenal of run and r un hydrate to protect plasmid DNA against damage induced by irradiaon. Materials and Methods: DPPH and hydroxyl radical scavenging assays were performed to assess the anradical potenal of run and run hydrate. Absor pon measurements were performed to assess binding parameters of run and run hydrate with calf thymus (CT)-DNA. Plasmid relaxaon assay was performed to compare the radio protecve potenal of run and run hydra te against gamma irradiaon mediated oxidave damage of pET28 plasmi d DNA. Results: DPPH ·