A study of cell transfer with mounting media.

Cell transfer is applicable to broken slides and the preparation of multiple slides from a single smear on limited cytological material. We clarified the effectiveness of cell transfer by different mounting media and used resulting preparations to conduct immunocytochemical staining. Our results indicate that temperature and time in softening were affected by the properties (viscosity and xylene components) of mounting media. Cellular preservation and reactivity of most antigens are maintained following transfer. Cytological materials prepared by cell transfer are therefore suitable for immunocytochemical study.