Abstract 11378: Dynamic Secretion Analysis by Novel Microfluidic Platform mFLISA Reveal Contextual and Precise Paracrine Signatures Preventing Cardiac Injury

It is now increasingly recognized that a variety of stem cells offer therapeutic benefit to injured tissues via paracrine signaling. Paracrine signaling via secreted ligands is an important mediator of cell function. It has been a challenge to precisely measure cellular secretions in relevant physiological contexts. Here we introduce a high throughput platform combining protein microprinting and microfluidics, μFLISA, to directly measure cellular secretion and estimate the kinetics for the detected secretome (Fig. 1A). μFLISA design allows for precise evaluation of the diffusion of secreted molecules, as well as retroactive determination of the dynamics of secretion without time course measurements (Fig. 1B). Furthermore, its unique architecture facilitates precise control over measurement timings in response to arbitrary biological stimuli. Using μFLISA we estimated the secretion dynamics for different constituents of the secretome for bone marrow derived stem cells (BMSCs), reported to provide benefit i...