Effects of Hemeoxygenase-1 on Oxygen-Glucose Deprived Hippocampus Neuron Mitochondrial Movement Protein Expression

Objective To investigate the effects of hemeoxygenase-1(HO-1)on mitochondrial movement protein expression in primary cultured rat hippocampus neurons after oxygen glucose deprivation.Methods Primary rat hippocumpus neurons cultrured for 7 days were dividend into four groups.Experimental group cells were respectively carried out 10μmol/L Heme precondition(group C+H),OGD(group D),10 μmol/L Heme precondition + OGD(group D+H),Control cells were cultured normally(group C).Compound remained present throughout the duration of the experiment until analysis 24 h later.Neuron viability and apoptosis were weasured.The expression of HO-1,Miro1,Miro2,Milton protein and HO-1-mRNA were detected.Results The protein and mRNA expression levels of HO-1 in group C+H were higher than group C(P0.01),the protein expression levels of Miro1,Miro2 and Milton were higher than group C(P0.01).The neuron viability and apoptosis had no significant difference between group C+H and group C(P0.05).The protein and mRNA expression levels of HO-1 in group D were higher than group C(P0.01),the protein expression levels of Miro1,Miro2 and Milton were higher than group C(P0.01),the neuron viability rate was lower and neuron apoptosis rate was higher than group C(P0.01).The protein and mRNA expression levels of HO-1 in group D+H were higher than group D(P0.01),the protein expression levels of Miro1,Miro2 and Milton were higher than group D(P0.01),the neuron viability rate was higher and neuron apoptosis rate was lower than group D(P0.01).Conclusion HO-1 can inhibit the apoptosis of hippocampus neurons induced by oxygen glucose deprivation through increasing mitochondrial movement proteins expression.