Short Communication Relationship between adenovirus DNA replication proteins and nucleolar proteins B23.1 and B23.2

Adenovirus infection subverts nucleolar structure and function. B23 is a nucleolar protein presentin two isoforms (B23.1 and B23.2) and both isoforms have been identified as stimulatory factorsfor adenovirus DNA replication. Here, it is demonstrated that the two isoforms of B23, B23.1and B23.2, interact and co-localize differently with viral DNA replication proteins pTP and DBP inadenovirus-infected cells. Thus, the mechanism by which the two proteins stimulate viral DNAreplication is likely to differ. These data also demonstrate the importance of testing both isoformsof B23 for interactions with viral proteins and nucleic acids.