Adriamycin intervention and the expression of adrenocortical carcinoma stem cell marks

Objective To explore the expression of CD133 and ABCG2 in adrenocortical carcinoma (ACC) xenograft treated by adriamycin in vivo,and to preliminarily analyze the significance.Methods Established the nude mice model of SW-13 transplanted subcutaneously,one group was intervened with ADM in vivo and obtained ADM group cells by primary culture,the other group was not intervened with ADM and obtained control group cells by primary culture.Cell proliferation capacity was examined by single-cell clone formation assay.The expression of CD133 and ABCG2 were detected by flow cytometry and immunohistochemistry.Results The colony formation rate of ADM group was stronger than that of control group (P ＜0.05).Flow cytometry and immunohistochemistry showed that ABCG2 were positive in both groups,the expression rate of ABCG2 by flow cytometry was higher in ADM group(P ＜ 0.05),but the two methods indicated CD133 was negative in both groups.Conclusions The ADM group cells have certain characteristics of cancer stem cells.ABCG2 is associated with the formation of drug resistance in ACC and may be a potential therapeutic target. Key words: Adrenocortical carcinoma;  Adriamycin;  ABCG2 ;  CD133