1,25-Dihydroxyvitamin D_3 reduces motility of podocytes via inhibiting urokinase-type plasminogen activator receptor expression

AIM To investigate the effects of 1,25-dihydroxyvitamin D_3(1,25(OH)_2D_3) on the lipopolysaecharide(LPS) -induced motility of podocytes and the expression of urokinase-type plasminogen activator receptor(uPAR) in vitro.METHODS Immortalized mouse podocytes were cultured and randomized into three groups:control group,LPS(50 mg·L~(-1)) group,LPS(50 mg·L~(-1)) +1,25(OH)_2D_3(1nmol·L~(-1)) group.The podocytes motility was checked by transwell migration assay.The expression of uPAR in podocytes were detected by immunofluorence method,flow cytometry method and real-time PCR.RESULTS The number of podocytes in the transwell migration assay were 8.4±3.8,31.3±7.9,19.2±6.8 in the control group,LPS group and LPS + 1,25(OH)_2D_3 group,respectively.The motility of LPS-induced podocytes in the LPS group were increased compared with the other two groups(P0.01).The immunofluorescence staining of uPAR protein in the LPS group was increased compared with those in the control group and LPS +1,25(OH)_2D_3 group.The positive expression rate of uPAR protein and the Plaur mRNA in the LPS + 1,25(OH)_2D_3 group ((31.12±4.46)%and 1.97±0.46) were lower than those in the LPS group((54.66±8.69)%and 3.06±0.93,P0.05),but higher than those in the control group((9.80±3.11)%and 1.00±0.00,P0.05). CONCLUSION 1,25(OH)_2D_3 could reduce LPS-induced motility of podocytes via inhibiting uPAR expression.