Reproduction of the vesicular stomatitis virus in mouse splenocytes

: Interaction of vesicular stomatitis virus with immunocompetent cells in vivo and in vitro was studied. In intact mice, a prolonged infection of the spleens with low levels of virus replication was observed. Experiments with various splenocyte cultures demonstrated differences in their capacity to support virus reproduction. A relationship between the functional activity of the cells (the level of spontaneous blastogenesis, index of PHA response) and levels of virus reproduction was shown. Mitogen stimulation of blastogenesis was accompanied by intensified replication in reactive cultures. In splenocyte cultures from immune animals, reproduction levels were decreased and virus replication stopped.