Thymine-modified chitosan with broad-spectrum antimicrobial activities for wound healing

Abstract Avoiding wound infections is a major challenge in wound care management, and new materials are urgently needed to address these problems. Herein, four water-soluble thymine-modified chitosan (TC) derivatives with the degree of substitution (DS) ranging from 0.23 to 0.62 were synthesized and freeze-dried to obtain porous sponge dressings. TC derivatives had broad-spectrum antibacterial activities against gram-negative bacteria, gram-positive bacteria, fungi, drug-resistance bacteria, Pseudomonas aeruginosa and Acinetobacter baumannii. Especially, the antioxidant and antibacterial properties of TC derivatives increased with increasing DS. Furthermore, TC derivatives showed excellent biocompatibility and blood compatibility. TC sponges could significantly accelerate the wound healing process than gauze and chitosan sponge. The histological analysis and immunohistochemical staining showed that the wounds treated with TC sponges displayed fewer inflammatory cells, and faster regeneration of epithelial tissue, collagen deposition and new blood vessel formation speed. Therefore, TC sponges can shed new light for wound dressing design.