A fusion gene and its preparation method, the fusion gene expression vector and its construction method, the fusion gene engineering bacteria and their application

The present invention discloses a method for preparing a fusion gene and the gene fusion expression vector and its construction method, the fusion gene engineering bacteria and their application, belongs to the technical field of genetic engineering. The fusion gene, the TAT protein transduction domain connected to the diuretic HKI polypeptide sequence, transmembrane transport and efficient means of action of TAT, HKI so quickly through the esophagus lining system into the body site, ensuring stable HKI play during transport in vivo the role of the cotton bollworm excessive excretion death. 3 fusion gene expression vector of the present invention, the fusion gene construct in the plant expression vector pB 121 Ⅰ, the expression driven by the 35S promoter, highly expressed in plants, feeding on transgenic plants bollworm inventive fusion expression vector of genetic transformation instar larvae of the cotton bollworm significantly reduced weight gain, high mortality. The present invention is fused genetically engineered bacteria, plants to be disseminated germination, genetic fusion to achieve transformed plants, insect-resistant transgenic plants bred.