Akkermansia muciniphila Prevents Fatty Liver, Decreases Serum Triglycerides, and Maintains Gut Homeostasis

The objective of this study was to elucidate the effect of intestinal Akkermansia muciniphila on fatty liver disease. Five-week-old C57BL/6N mice were administered either phosphate buffer saline (PBS, control) or A. muciniphila at 108-109 CFU/mL, and were fed either a 45% fat diet (HFD, high-fat diet) or a 10% fat diet (ND) for 10 weeks. After 10 weeks, the mice were euthanized, and the blood and tissue samples, including adipose tissue, caecum, liver, and brain, were immediately collected. Biochemical and histological analyses were conducted and the expression levels of related factors were compared to determine the anti-obesity effects of Akkermansia muciniphila. The gut microbiome was analyzed in fecal samples. Oral administration of A. muciniphila significantly (p Importance This study investigated the effect of Akkermansia muciniphila on fatty liver disease. Though some researches about effects of A. muciniphila on host health were published, the study on relation between A. muciniphila administration and fatty liver as well as changes of gut microbiota have not been conducted. In this study we specifically focused on composition of gut microbiota, and also demonstrate gene expression levels, regulating fat synthesis and inflammation in the liver.