A Generic High-Throughput Screening Assay for Kinases: Protein Kinase A as an Example
2003
** † 33 P-peptide product is captured onto polylysine Ysi bead via avidin. The scintillation signal measuring the product formation increases linearly with avidin concentration due to effective capture of the product on the bead surface via strong coulombic interactions. This novel assay has been optimized and validated in 384-well
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