Enhancement of A23187-induced release of enteric vasoactive intestinal polypeptide by phorbol 12,13-dibutyrate.

Abstract The effect of phorbol 12,13-dibutyrate on calcium ionophore (A23187)-induced release of vasoactive intestinal polypeptide from enteric nerves was examined. A23187 caused release of vasoactive intestinal polypeptide dose dependently and phorbol 12,13-dibutyrate (100 nM) markedly shifted the dose-response curve of A23187 to the left. The significance of these findings will be discussed in relation to the mechanism of release of vasoactive intestinal polypeptide from enteric nerves.