Rapid Determination of Chlorogenic Acid,Neochlorogenic Acid and Rutin in Tobacco with NIR Spectroscopy

In order to determine chlorogenic acid,neochlorogenic acid and rutin in tobacco rapidly,the near infrared spectra of 183 tobacco samples were measured with near infrared spectroscopy and the three compounds in samples were determined by HPLC.The calibration models between spectral data and the contents of chlorogenic acid,neochlorogenic acid and rutin were developed by preprocessing spectra with multiplicative scatter calibration,first derivative + scatter calibration,and then the processed spectra and the contents of the three compounds were regressed by partial least square.The models were verified by internal cross validation and external predictive validation with 30 tobacco samples.The results showed that the average relative errors between NIR-predicted values and determined values were 0.545%,0.709% and 1.954% with RSDs of 0.83%,3.18% and 1.426%,respectively.This method was suitable for the rapid determination of chlorogenic acid,neochlorogenic acid and rutin in batches of tobacco samples.