Динамика изменений иммунофенотипа В-лимфоцитов на различных стадиях хронического лимфолейкоза, их диагностическое и прогностическое значения

The research work presents results of clinical laboratory investigations of dynamic changes of immune phenotype of B-lymphocytes in different stages of B-cell mode of chronic lymphocytic leukemia. The detection of the content of CD-marker molecules in 60 patients with different stages of B-cell mode of chronic lymphocytic leukemia (0-I, II, III, IV according K.R. Rai et al. classification, 1975) was conducted. Investigation of the character of expression of marker molecules (CD19, CD5, CD20, CD23, CD38) on B-lymphocytes of peripheral blood with using of standard panel of monoclonal antibodies for flow cytometry «Facs-Calibur» (BD, USA, 2006) was carried out. The examination of patients with this pathology reveals some regularities of changes of immune phenotype of B-lymphocytes which were parallel with stages of the disease. The appearance of atypia of B-lymphocytes characterized by co-expression of CD19 and CD5 as well as significant activation of B-cells characterized by expression of CD23 and CD38 were observed.