UV-Spectrophotometric method for quantification of ascorbic acid in bulk powder

Ascorbic acid is a potent reducing and antioxidant agent that functions in fighting bacterial infections, in detoxifying reactions. In the present research work an attempt has been made to develop and validate spectrophotometric method for determination of ascorbic acid in bulk powder form using UV-1900 model. The method was developed using methanol: water (50:50 v/v) as a solvent in which ascorbic acid shows maximum absorbance wavelength at 258nm. In order to optimize the developed method, validation of ascorbic acid were performed as per ICH guidelines parameters such as Linearity, Selectivity, Specificity, Precision, Robustness, Ruggedness, LOD & LOQ, Solution stability were performed. Ascorbic acid Showed linear response between concentration range of 3, 6, 9, 12, 15µg/mL with r2 value 0.997 all the validation report of Precision, robustness, ruggedness and solution stability were found to be in acceptance limit. Newly developed and validated spectrophotometric method was found to be selective, specific, linear, precise, robust, rugged and stable for determination of ascorbic acid in bulk form using UV-1900.