Analysis of volatile compounds in beer by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometer

Volatile compounds in beer was analyzed using comprehensive two dimensional gas chromatography/time-of-flight mass spectrometer(GC×GC/TOFMS)．In total, more than 300 compounds in beer were identified with good match and similarity and reverse number over 750 ~ 900.The main volatile compounds in beer were detected by this method,, such as Hexanoic acid, ethy1 ester /3-Buten-1-one, 4-[2,6,6-trimethyl-1(or2)-cyclohexen-1-yl]-/ Cedrol/ Phenylethyl Alcohol/ Benzyl Benzoate/Limonene/Pinene/1,3,7-Octatriene, 3,7-dimethyl-/2-Buten-1-one,1-(2,6,6-trimethyl-1, 3-cyclohexadien-1-yl)-,[E]- /Octanoic acid, ethyl ester /Decanoic acid, ethyl ester/ Tetradecanoic acid,ethyl ester/ Pentadecanoic acid, ethyl ester / Acetic acid, 2-phenylethyl ester/ Benzoic acid, butyl ester / Naphthalene, 1-methyl-/ Benzenepropanoic acid, ethyl ester / Butylated Hydroxytoluene /Phenylethyl Alcohol / 1,6,10-Dodecatrien-3-ol, 3,7,11-trimethyl-,[E]- /Tetradecanoic acid, ethyl ester / 2(3H)-Furanone, dihydro-5-pentyl- / 2-Propenoic acid, 3-phenyl-, ethyl ester ,etc..(fig1) In classical methods, the beer sample was also quantified by one-dimensional gas chromatography-mass spectrometry (GC/MS),and we find about 30constituents.The results showed that we can obtained more volatile constituents by GC×GC-TOF/MS than GC/MS analysis methods. The results is very useful for disclosing the relationship between components and the aroma of beer, and helpful for beer data base to controlling high quality products. Fig1 Volatile compounds in beer was analyzed using GC×GC/TOFMS