enzymatic processing antibody

Method for producing an immunoglobulin or polypeptide comprising at least the CH2 domain and the CH3 domain of a immunoglobulin heavy chain full length with a defined glucoestructura, comprising the following steps: - providing an eluate of column affinity chromatography containing the immunoglobulin or immunoglobulin fragment, - incubating the eluted column affinity chromatography with an α-galactosidases of plant origin that cleaves terminal galactose residues linked by link (α1,3) -glucosidico in the nonreducing end of the glucoestructura in the CH2 domain of the immunoglobulin or immunoglobulin fragment, - applying the eluate from column affinity chromatography incubated a material protein a chromatography and recovering the immunoglobulin or immunoglobulin fragment from material protein a chromatography and thereby produce an immunoglobulin or fragment inmunog lobulina with glucoestructura defined.