HPLC를 이용한 조제유류 중 베타카로틴 함량 분석 연구

A procedure based on high-performance liquid chromatography (HPLC) is described to determine β-carotene in infant formulas. The method for β-carotene analysis was performed on a C18 reversed-phase column using acetonitrile/methanol/dichloromethane (6:1:3, v/v/v) as a mobile phase. β-Carotene was determined in HPLC with photo diode array (PDA) detector. The parameters of validation were specificity, linearity, LOD, LOQ, accuracy, precision and repeatability. The specificity was confirmed by the retention time and the linearity (R2), which was over 0.999 in the range of 0.125~2 mg/L. The detection and quantification limits were 0.064 and 0.193 mg/L, respectively. The accuracy and precision of this method using an STD spiked sample were 80~119% and 1.02~2.05% respectively. The method was applied to the analysis of various infant formula and follow-up formulas products containing β-carotene, and all the products contained acceptable levels of β-carotene for nutrition labeling.