Qualitative Triplex PCR for the Detection of Genetically Modified Soybean and Maize

ABSTRACTA molecular screening method based on multiplex PCR that involves amplification of specific soybean or maize sequences from plant DNA (lectin or zein) and the amplification of 35S promoter and NOS terminator for the detection of genetically modified soybean and maize was developed. The new method is proposed for the simultaneous detection of tree genetic elements in the same run as reliable method for rapid detection of genetically modified plants with sensitivity of 0.1%.