1304 – Histochemical study of substance cholecystokinin immunoreactive neurons in the cortex of human inferion parietal lobule and their importance in the clinic

Introduction Cholecystocinine is a neuropeptide which function in cortex has not yet clarified, although its relation with some psychic disorders has been noticed. Aim The aim of this study was a precise examination of morphology and typography of neurons which contain cholecytocinine in human cortex of inferior parietal lobule. Material and methods There were six human brains on which we did the immunocystochemical research of shape and laminar distribution of cholecystocinine immunoreactive neurons on serial sections of supramarginal gyrus and angular gyrus. Morphological analysis of neurons whicha are immunoreactive to cholecystocinine is done on frozen sections using avidin- biotin technique, by antibody to cholecystocinine diluted in proportion 1:6000 using diamine-benzedine. Results Neurons that are immunorective to cholecystocinine were found in the first three layers of cortex inferior parietal lobule, and their densest concentration was in the 2nd and 3rd layer. The following types of neurons were found: bipolar neurons, then its fusiform subtype, Cajal-Retzius neurons (in the 1st layer), reverse pyramidal (triangular) and unipolar neurons. The diameters of some types of neurons were from 10 to 28 μm, and the diameters of dendritic arborization were from 80-201 μm. Conclusion Regarding the numerous clinical researches, in which it has clearly been evinced about the role of cholecystokinine in pathogenesis of schizophrenia, and according to the findings of the great number of neurons, which are immunoreactive to cholecystokinine in cortex of inferior parietal lobule, it is suggested that cholecystokinine has a significant role in pathogenesis of schizophrenia.