Abstract 16220: Negative Regulation of Mir-375 by Interleukin-10 Enhances Endothelial Progenitor Cell Mediated Myocardial Repair and Function After Myocardial Infarction

Despite some encouraging data, the bone marrow progenitor cells (BMPC) mediated clinical trials have only shown a very modest improvement in heart functions. Poor survival and function of transplanted cell in the ischemic and inflamed myocardium likely compromises the full functional benefit of stem cell based therapies. We have earlier reported that co-administration of IL-10 and BMPC not only enhances cell survival but also improved left ventricular (LV) functions after AMI in mice. We identified that microRNA-375 expression is significantly up-regulated in EPCs upon exposure to either inflammatory or hypoxic stimulus. We hypothesized that IL-10 regulates miR-375 signaling in EPCs to enhance their survival and function in ischemic myocardium after MI. miR-375 knock down EPC were transplanted intramyocardially after induction of MI. Mice receiving EPC treated with miR-375 inhibitor showed increased number of EPC retention that was associated with reduced EPC apoptosis in the myocardium. The engraftment o...